Sex determination of cattle meat by polymerase chain reaction amplification of the amelogenin (AMELX/AMELY) gene

Sex determination of cattle meat by polymerase chain reaction amplification of the amelogenin (AMELX/AMELY) gene

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Aim: The objective of this study was to develop a simplified, efficient, and accurate protocol for sexing of cattle meat based on the amelogenin (AMELX/AMELY) gene using PCR technique which is superior weboost splitter to earlier work in terms of band patterns.Materials and Methods: Based on the amelogenin gene located on the conservation region of X and Y chromosomes, a pair of primers was designed and the system of PCR was established to amplify a 241-bp fragment from the X chromosome in female cattle, and a 241-bp fragment from X chromosome and 178-bp fragment from the Y chromosome in male cattle, respectively.The accuracy and specificity of read more the primers was assessed using DNA template extracted from cattle meat samples of known sex.

The protocol was subjected to a blind test showed 100% concordance, proving its accuracy and reliability.Results: PCR products of cattle meat samples after electrophoresis showed two bands (241, 178-bp) for tissue from male while female tissue resulted in only one (241-bp) band.Conclusions: Our findings show that the PCR assay based on the amelogenin gene is reliable for sex determination in cattle meat.

[Vet World 2012; 5(9.000): 526-529].